Electromembrane extraction of streptomycin from biological fluids
نویسندگان
چکیده
In this fundamental study, streptomycin was extracted successfully from urine and plasma using electromembrane extraction (EME). Streptomycin is an aminoglycoside with log P -7.6 selected as extremely polar model analyte. EME a microextraction technique, where charged analytes are under the influence of electrical field, sample, through supported liquid membrane (SLM), into acceptor solution. The SLM comprised 2-nitrophenyl pentyl ether (NPPE) mixed bis(2-ethylhexyl) phosphate (DEHP). DEHP served ionic carrier facilitated transfer across SLM. For protein precipitated plasma, optimal content in 45–50% w/w. From untreated increased to 75% w/w order suppress interference proteins. Most endogenous substances UV absorbance were not acceptor. Proteins phospholipids also discriminated, <0.6% proteins <0.02% found after EME. Thus, despite fact that permeable more molecules, still provided very efficient sample cleanup. Extraction process efficiencies 98% 61% achieved respectively, linear calibration (R2 > 0.9929), absence significant matrix effects (94–112%), accuracy 94–125%, RSD ? 15% except at LLOQ. average current during extractions 67 µA or less. findings paper demonstrated feasible for basic extreme polarity.
منابع مشابه
Electromembrane extraction from biological fluids.
Electro-assisted extraction of ionic drugs from biological fluids through a supported liquid membrane (SLM) and into an aqueous acceptor solution was recently introduced as a new sample preparation technique termed electromembrane extraction (EME). The applied electrical potential across the SLM has typically been in the range of 1-300 V. Successful extractions have been demonstrated even with ...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2021
ISSN: ['1873-3778', '0021-9673']
DOI: https://doi.org/10.1016/j.chroma.2021.461915